Anti-Bcr Antibody Picoband®

About this product

Boster Bio Anti-Bcr Antibody Picoband® catalog # PB9988. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9988

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The breakpoint cluster region protein (BCR) is a protein that in humans is encoded by the BCR gene. A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Breakpoint cluster region protein;2.7.11.1; Renal carcinoma antigen NY-REN-26; BCR; BCR1, D22S11

Gene Name

BCR

Gene ID

613

UniProt

P11274

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Bcr recombinant protein (Position: M1-A100) . Human Bcr shares 94% amino acid (aa) sequence identity with mouse Bcr.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous, with higher levels in heart, substantia nigra, and kidney. .

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Cancer, G Protein Signaling, Protein Phosphorylation, Ras Family, Ser/Thr Kinases, Serine/Threonine Kinases, Signal Transduction, Signaling Pathway, Small G Proteins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity. .

References & Citations

1. Entrez Gene: Breakpoint cluster region. 2. Puil L, Liu J, Gish G, Mbamalu G, Bowtell D, Pelicci PG, Arlinghaus R, Pawson T (February 1994) . Bcr-Abl oncoproteins bind ly to activators of the Ras signalling pathway. EMBO J. 13 (4) : 764–73. 3. Pendergast AM, Muller AJ, Havlik MH, Maru Y, Witte ON (July 1991) . BCR sequences essential for transformation by the BCR-ABL oncogene bind to the ABL SH2 regulatory domain in a non-phosphotyrosine-dependent manner.Cell 66 (1) : 161–71.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

142819 MW

Observed Molecular Weight

143 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Breakpoint cluster region protein

Subcellular Location

Cell junction, synapse, postsynaptic cell membrane, postsynaptic density .

Protein Name

Breakpoint cluster region protein

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.