Anti-Carbonic Anhydrase II/CA2 Antibody Picoband®

About this product

Boster Bio Anti-Carbonic Anhydrase II/CA2 Antibody Picoband® catalog # PB9989. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9989

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CA2 is a cytosolic enzyme with the highest activity among all known CAs. The carbonic anhydrases (ACs) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs relatively slowly in the absence of a catalyst. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. This gene is mapped to 8q22.

Synonyms

Carbonic anhydrase 2;4.2.1.1; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II; CA2

Gene Name

CA2

Gene ID

760

UniProt

P00918

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CA2 recombinant protein (Position: S2-K260) . Human CA2 shares 81.1% and 80.7% amino acid (aa) sequence identity with mouse and rat CA2, respectively.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous, with higher levels in heart, substantia nigra, and kidney. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Alzheimer's Disease, Biochemicals, Cancer, Chemical Type, Diabetes, Heart Disease, Microbiology, Obesity

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Essential for bone resorption and osteoclast differentiation (By similarity) . Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. .

References & Citations

1. Aramaki, S., Yoshida, I., Yoshino, M., Kondo, M., Sato, Y., Noda, K., Jo, R., Okue, A., Sai, N., Yamashita, F. Carbonic anhydrase II deficiency in three unrelated Japanese patients. J. Inherit. Metab. Dis. 16: 982-990, 1993. 2. Laitala, T., Vaananen, H. K. Inhibition of bone resorption in vitro by antisense RNA and DNA molecules targeted against carbonic anhydrase II or two subunits of vacuolar H (+) -ATPase. J. Clin. Invest. 93: 2311-2318, 1994. 3. Venta, P. J., Welty, R. J., Johnson, T. M., Sly, W. S., Tashian, R. E. Carbonic anhydrase II deficiency syndrome in a Belgian family is caused by a point mutation at an invariant histidine residue (107his-to-tyr) : complete structure of the normal human CA II gene. Am. J. Hum. Genet. 49: 1082-1090, 1991.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

29246 MW

Observed Molecular Weight

29 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Carbonic anhydrase 2

Subcellular Location

Cytoplasm . Cell membrane . Colocalized with SLC26A6 at the surface of the cell membrane in order to form a bicarbonate transport metabolon. Displaced from the cytosolic surface of the cell membrane by PKC in phorbol myristate acetate (PMA) -induced cells.

Protein Name

Carbonic anhydrase 2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.