Anti-Cyclin T1/CCNT1 Antibody Picoband®

About this product

Boster Bio Anti-Cyclin T1/CCNT1 Antibody Picoband® catalog # PB9990. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9990

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cyclin-T1 is a protein that in humans is encoded by the CCNT1 gene. This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb) . In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants.

Synonyms

Cyclin-T1; CycT1; Cyclin-T; CCNT1

Gene Name

CCNT1

Gene ID

904

UniProt

O60563

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Cyclin T1, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Antiviral Signaling, Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Host-Virus Interaction, Immune System Diseases, Immunology, Interspecies Interaction, Microbiology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II) . .

References & Citations

1. Entrez Gene: CCNT1 cyclin T1. 2. Peng J, Zhu Y, Milton JT, Price DH (April 1998) . Identification of multiple cyclin subunits of human P-TEFb. Genes Dev. 12 (5) : 755–62. 3. Wei P, Garber ME, Fang SM, Fischer WH, Jones KA (March 1998) . A novel CDK9-associated C-type cyclin interacts ly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA. Cell92 (4) : 451–62.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

80685 MW

Observed Molecular Weight

81 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cyclin-T1

Subcellular Location

Nucleus .

Protein Name

Cyclin-T1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.