Anti-Carboxypeptidase B2/CPB2 Antibody Picoband®

About this product

Boster Bio Anti-Carboxypeptidase B2/CPB2 Antibody Picoband® catalog # PB9997. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9997

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

Synonyms

Carboxypeptidase B2;3.4.17.20 ; Carboxypeptidase U; CPU; Plasma carboxypeptidase B; pCPB; Thrombin-activable fibrinolysis inhibitor; TAFI; CPB2

Gene Name

CPB2

Gene ID

1361

UniProt

Q96IY4

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human CPB2 recombinant protein (Position: K166-D388) . Human CPB2 shares 86.1% and 85.7% amino acid (aa) sequence identity with mouse and rat CPB2, respectively.

Clonality

Polyclonal

Tissue Specificity

Plasma; synthesized in the liver.

Applications

ELISA (Cap), IHC, WB

Field of Research

Amino Acid Metabolism, Amino Acids, Atherosclerosis, Cardiovascular, Heart Disease, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. .

References & Citations

1. Boffa MB, Reid TS, Joo E, Nesheim ME, Koschinsky ML (May 1999) . Characterization of the gene encoding human TAFI (thrombin-activable fibrinolysis inhibitor; plasma procarboxypeptidase B) . Biochemistry 38 (20) : 6547–58. 2. Eaton DL, Malloy BE, Tsai SP, Henzel W, Drayna D (Dec 1991) . Isolation, molecular cloning, and partial characterization of a novel carboxypeptidase B from human plasma. J Biol Chem 266 (32) : 21833–8. 3. Tsai SP, Drayna D (Dec 1992) . The gene encoding human plasma carboxypeptidase B (CPB2) resides on chromosome 13. Genomics14 (2) : 549–50.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48424 MW

Observed Molecular Weight

48 kDa, 60 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Carboxypeptidase B2

Subcellular Location

Secreted.

Protein Name

Carboxypeptidase B2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.