Anti-BCA1/CXCL13 Antibody Picoband®

About this product

Boster Bio Anti-BCA1/CXCL13 Antibody Picoband® catalog # PB9999. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9999

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

C-X-C motif chemokine 13 (CXCL13), also known as B cell-attracting chemokine 1 (BCA-1), is a protein that in humans is encoded by the CXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family. Its gene is mapped to human chromosome 4 in a cluster of other CXC chemokines. This protein has contained 109 amino acids. It is expressed highly in the liver, spleen, lymph nodes and gut of humans. The protein induces chemotaxis of BLR1-expressing B cells.

Synonyms

C-X-C motif chemokine 13; B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13; Cxcl13; Blc, Scyb13

Gene Name

Cxcl13

Gene ID

55985

UniProt

O55038

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109) . Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1.

Clonality

Polyclonal

Tissue Specificity

Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles) .

Applications

ELISA, IHC, WB

Field of Research

Cardiovascular, Heart Disease, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in ing the migration of B-lymphocytes to follicles in secondary lymphoid organs.

References & Citations

1. Ansel, K. M., Harris, R. B. S., Cyster, J. G. CXCL13 is required for B1 cell homing, natural antibody production, and body cavity immunity. Immunity 16: 67-76, 2002. 2. Gunn MD, Ngo VN, Ansel KM, Ekland EH, Cyster JG, Williams LT (February 1998) . A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1. Nature 391 (6669) : 799–803. 3. Legler DF, Loetscher M, Roos RS, Clark-Lewis I, Baggiolini M, Moser B (February 1998) . B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5. J. Exp. Med. 187 (4) : 655–60.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

12 kDa

Observed Molecular Weight

11 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

C-X-C motif chemokine 13

Subcellular Location

Secreted.

Protein Name

C-X-C motif chemokine 13

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.