Anti-DDAH1 Antibody Picoband®

About this product

Boster Bio Anti-DDAH1 Antibody Picoband® catalog # PB10000. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10000

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

DDAH1 is knowns as dimethylarginine dimethylaminohydrolase 1 which is mapped to chromosome 1p22 by radiation hybrid and FISH analysis. This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. It widely expressed, especially in liver and kidney.

Synonyms

N (G), N (G) -dimethylarginine dimethylaminohydrolase 1; DDAH-1; Dimethylarginine dimethylaminohydrolase 1;3.5.3.18; DDAHI; Dimethylargininase-1; DDAH1; DDAH

Gene Name

DDAH1

Gene ID

23576

UniProt

O94760

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DDAH1, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cardiovascular, Neuroscience, Neurotransmission, Vasculature

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Hydrolyzes N (G), N (G) -dimethyl-L-arginine (ADMA) and N (G) -monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.

References & Citations

1. Dayoub, H., Achan, V., Adimoolam, S., Jacobi, J., Stuehlinger, M. C., Wang, B., Tsao, P. S., Kimoto, M., Vallance, P., Patterson, A. J., Cooke, J. P. Dimethylarginine dimethylaminohydrolase regulates nitric oxide synthesis: genetic and physiological evidence.Circulation 108: 3042-3047, 2003. 2. Millatt, L. J., Whitley, G. StJ., Li, D., Leiper, J. M., Siragy, H. M., Carey, R. M., Johns, R. A. Evidence for dysregulation of dimethylarginine dimethylaminohydrolase I in chronic hypoxia-induced pulmonary hypertension. Circulation 108: 1493-1498, 2003. 3. Tran, C. T. L., Fox, M. F., Vallance, P., Leiper, J. M. Chromosomal localization, gene structure, and expression pattern of DDAH1: comparison with DDAH2 and implications for evolutionary origins. Genomics 68: 101-105, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

31122 MW

Observed Molecular Weight

31 kDa, 37 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

N (G), N (G) -dimethylarginine dimethylaminohydrolase 1

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Protein Name

N (G), N (G) -dimethylarginine dimethylaminohydrolase 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.