Anti-Alpha Internexin/INA Antibody Picoband®

About this product

Boster Bio Anti-Alpha Internexin/INA Antibody Picoband® catalog # PB10003. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10003

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alpha-Internexin (INA; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. The protein was originally purified from rat optic nerve and spinal cord. And the protein copurifies with other neurofilament subunits, as it was originally discovered, however in some mature neurons it can be the only neurofilament expressed. The protein is present in developing neuroblasts and in the Central Nervous System of adults. Meanwhile, the protein is a major component of the intermediate filament network in small interneurons and cerebellar granule cells, where it is present in the parallel fibers.

Synonyms

Alpha-internexin; Alpha-Inx;66 kDa neurofilament protein; NF-66; Neurofilament-66; Neurofilament 5; INA; NEF5

Gene Name

INA

Gene ID

9118

UniProt

Q16352

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Alpha Internexin recombinant protein (Position: A71-R161) . Human Alpha Internexin shares 98.9% amino acid (aa) sequence identity with both mouse and rat Alpha Internexin.

Clonality

Polyclonal

Tissue Specificity

Found predominantly in adult CNS.

Applications

IF, IHC, ICC, WB, Flow Cytometry

Field of Research

Cardiovascular, Complement, Immunology, Innate Immunity

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.

References & Citations

1. Chan, S.-O., Chiu, F.-C. Cloning and developmental expression of human 66 kd neurofilament protein. Molec. Brain Res. 29: 177-184, 1995. 2. Levavasseur F, Zhu Q, and JP Julien. No requirement of alpha-internexin for nervous system development and for radial growth of axons. Molecular Brain Research. 69:104-112. (1999) .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

55391 MW

Observed Molecular Weight

62-66 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Alpha-internexin

Subcellular Location

Nucleus . Chromosome . Cytoplasm . Secreted . In basal state predominantly nuclear. .

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Protein Name

Alpha-internexin

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.