Anti-Visfatin/NAMPT Antibody Picoband®

About this product

Boster Bio Anti-Visfatin/NAMPT Antibody Picoband® catalog # PB10009. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10009

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme that in humans is encoded by the PBEF1 gene. This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.

Synonyms

Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt;2.4.2.12; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin; NAMPT; PBEF, PBEF1

Gene Name

NAMPT

Gene ID

10135

UniProt

P43490

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human Visfatin recombinant protein (Position: L62-N337) . Human Visfatin shares 97.1% and 96.7% amino acid (aa) sequence identity with mouse and rat Visfatin, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.

Applications

ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Adaptive Immunity, B Cells, Cancer, Cardiovascular, Cytokines, Growth Factors/Hormones, Hypoxia, Immunology, Innate Immunity, Metabolism, Metabolism Processes, Neurology Process, Neuroscience, Non-CD, Pathways and Processes, Serum Proteins, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the condensation of nicotinamide with 5- phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity) . .

References & Citations

1. Entrez Gene: PBEF1 pre-B-cell colony enhancing factor 1. 2. Samal B, Sun Y, Stearns G, Xie C, Suggs S, McNiece I (February 1994) . Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor. Mol. Cell. Biol. 14 (2) : 1431–7. 3. Wang T, Zhang X, Bheda P, Revollo JR, Imai S, Wolberger C (July 2006) . Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme. Nat. Struct. Mol. Biol. 13 (7) : 661–2.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

55521 MW

Observed Molecular Weight

54 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Nicotinamide phosphoribosyltransferase

Subcellular Location

Nucleus . Cytoplasm . Secreted . Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum. .

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Protein Name

Nicotinamide phosphoribosyltransferase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.