Anti-Mast Cell Tryptase/TPSAB1 Antibody Picoband®

About this product

Boster Bio Anti-Mast Cell Tryptase/TPSAB1 Antibody Picoband® catalog # PB10016. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10016

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tryptase alpha-1 and tryptase beta-1 are enzymes that in humans are encoded by the same TPSAB1 gene. Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. In addition, these genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

Synonyms

Tryptase alpha/beta-1; Tryptase-1;3.4.21.59; Tryptase I; Tryptase alpha-1; TPSAB1; TPS1, TPS2, TPSB1

Gene Name

TPSAB1

Gene ID

7177

UniProt

Q15661

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human Mast Cell Tryptase recombinant protein (Position: H65-P275) . Human Mast Cell Tryptase shares 77% and 76.1% amino acid (aa) sequence identity with mouse and rat Mast Cell Tryptase, respectively.

Clonality

Polyclonal

Tissue Specificity

Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level) . .

Applications

ELISA (Cap), IHC, WB

Field of Research

Amino Acid Metabolism, Amino Acids, Cancer, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315) . .

References & Citations

1. Entrez Gene: TPSAB1 tryptase alpha/beta 1. 2. Miller JS, Moxley G, Schwartz LB (Oct 1990) . Cloning and characterization of a second complementary DNA for human tryptase. J Clin Invest 86 (3) : 864–70. 3. Pallaoro M, Fejzo MS, Shayesteh L, Blount JL, Caughey GH (Feb 1999) . Characterization of genes encoding known and novel human mast cell tryptases on chromosome 16p13.3. J Biol Chem 274 (6) : 3355–62.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30515 MW

Observed Molecular Weight

30 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Tryptase alpha/beta-1

Subcellular Location

Secreted. Released from the secretory granules upon mast cell activation. .

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Protein Name

Tryptase alpha/beta-1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.