- Catalog number
- PB10017
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Vesicle-associated membrane protein 7 (VAMP-7), also known as SYBL1, is a protein that in humans is encoded by the VAMP7 gene. This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide- sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.
- Synonyms
- Vesicle-associated membrane protein 7; VAMP-7; Synaptobrevin-like protein 1; Tetanus-insensitive VAMP; Ti-VAMP; VAMP7; SYBL1
- Gene Name
- VAMP7
- Gene ID
- 6845
- UniProt
- P51809
- Host
- Rabbit
- Reactivity
- Human, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- E. coli-derived human SYBL1 recombinant protein (Position: N24-T190) . Human SYBL1 shares 97.6% and 95.2% amino acid (aa) sequence identity with mouse and rat SYBL1, respectively.
- Clonality
- Polyclonal
- Tissue Specificity
- Detected in all tissues tested.
- Applications
- WB
- Field of Research
- Protein Trafficking, Signal Transduction, Vesicle Transport
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. .
- References & Citations
- 1. Entrez Gene: SYBL1 synaptobrevin-like 1. 2. D'Esposito M, Ciccodicola A, Gianfrancesco F, Esposito T, Flagiello L, Mazzarella R, Schlessinger D, D'Urso M (Jul 1996) . A synaptobrevin-like gene in the Xq28 pseudoautosomal region undergoes X inactivation. Nat. Genet. 13 (2) : 227–9. 3. Filippini F, Rossi V, Galli T, Budillon A, D'Urso M, D'Esposito M (Jul 2001) . Longins: a new evolutionary conserved VAMP family sharing a novel SNARE domain. Trends Biochem. Sci. 26 (7) : 407–9.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 24935 MW
- Observed Molecular Weight
- 25 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Vesicle-associated membrane protein 7
- Subcellular Location
- Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass type IV membrane protein . Golgi apparatus, trans-Golgi network membrane ; Single-pass type IV membrane protein . Late endosome membrane ; Single-pass type IV membrane protein . Lysosome membrane ; Single-pass type IV membrane protein . Endoplasmic reticulum membrane ; Single-pass type IV membrane protein . Cytoplasmic vesicle, phagosome membrane ; Single-pass type IV membrane protein . Cell junction, synapse, synaptosome . In immature neurons expression is localized in vesicular structures in axons and dendrites while in mature neurons it is localized to the somatodendritic region. Colocalizes with LAMP1 in kidney cells. Localization to the endoplasmic reticulum membrane was observed in the intestine but not in liver or kidney (By similarity) . .
- Sequence Similarities
- Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.
- Protein Name
- Vesicle-associated membrane protein 7
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
