Anti-ABAT Antibody Picoband®

About this product

Boster Bio Anti-ABAT Antibody Picoband® catalog # PB10019. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10019

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

4-Aminobutyrate aminotransferase is a protein that in humans is encoded by the ABAT gene. ABAT is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5- phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of humans ynapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractoryseizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.

Synonyms

4-aminobutyrate aminotransferase, mitochondrial;2.6.1.19; (S) -3-amino-2-methylpropionate transaminase;2.6.1.22; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; GABA-T; L-AIBAT; ABAT; GABAT

Gene Name

ABAT

Gene ID

18

UniProt

P80404

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human ABAT recombinant protein (Position: K388-K500) . Human ABAT shares 93.9% and 94.5% amino acid (aa) sequence identity with mouse and rat ABAT, respectively.

Clonality

Polyclonal

Tissue Specificity

Liver > pancreas > brain > kidney > heart > placenta.

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Amino Acid Metabolism, Amino Acids, Cancer, Cancer Metabolism, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Neuroscience, Neurotransmitter, Pathways and Processes, Response To Hypoxia, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the conversion of gamma-aminobutyrate and L- beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.

References & Citations

1. De Biase D, Barra D, Simmaco M, John RA, Bossa F (Jan 1995) . Primary structure and tissue distribution of human 4-aminobutyrate aminotransferase. European Journal of Biochemistry / FEBS 227 (1-2) : 476–80. 2. Medina-Kauwe LK, Tobin AJ, De Meirleir L, Jaeken J, Jakobs C, Nyhan WL, Gibson KM (Jun 1999) . 4-Aminobutyrate aminotransferase (GABA-transaminase) deficiency.Journal of Inherited Metabolic Disease 22 (4) : 414–27.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

56439 MW

Observed Molecular Weight

54 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

4-aminobutyrate aminotransferase, mitochondrial

Subcellular Location

Mitochondrion matrix.

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Protein Name

4-aminobutyrate aminotransferase, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.