Anti-Abhd5 Antibody Picoband®

About this product

Boster Bio Anti-Abhd5 Antibody Picoband® catalog # PB10021. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10021

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

1-acylglycerol-3-phosphate O-acyltransferase ABHD5 is an enzyme that in humans is encoded by the ABHD5 gene. The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated withChanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

Synonyms

1-acylglycerol-3-phosphate O-acyltransferase ABHD5;2.3.1.51; Abhydrolase domain-containing protein 5; Lipid droplet-binding protein CGI-58; ABHD5; NCIE2; CGI-58

Gene Name

ABHD5

Gene ID

51099

UniProt

Q8WTS1

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human Abhd5 recombinant protein (Position: R169-D349) . Human Abhd5 shares 96.7% amino acid (aa) sequence identity with both mouse and rat Abhd5.

Clonality

Polyclonal

Tissue Specificity

Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level) . .

Applications

IF, IHC, ICC, WB

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Obesity, Pathways and Processes, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis (PubMed:18606822) . May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed:16679289) . Involved in keratinocyte differentiation (PubMed:18832586) . Regulates lipid droplet fusion (By similarity) . .

References & Citations

1. Ghosh AK, Ramakrishnan G, Chandramohan C, Rajasekharan R (Sep 2008) . CGI-58, the causative gene for Chanarin-Dorfman syndrome, mediates acylation of lysophosphatidic acid. The Journal of Biological Chemistry 283 (36) : 24525–33. 2. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011) . A conditional knockout resource for the genome-wide study of mouse gene function. Nature 474 (7351) : 337–342.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39096 MW

Observed Molecular Weight

43 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

1-acylglycerol-3-phosphate O-acyltransferase ABHD5

Subcellular Location

Cytoplasm . Lipid droplet . Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA (By similarity) . .

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Protein Name

1-acylglycerol-3-phosphate O-acyltransferase ABHD5

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.