Anti-AK2 Antibody Picoband®

About this product

Boster Bio Anti-AK2 Antibody Picoband® catalog # PB10034. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10034

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Adenylate kinase 2 is an enzyme is encoded in humans by the AK2 gene. The AK2 protein is found in the intermembrane space of the mitochondrion. Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.

Synonyms

Adenylate kinase 2, mitochondrial ; AK 2 ;2.7.4.3 ; ATP-AMP transphosphorylase 2 ; ATP:AMP phosphotransferase ; Adenylate monophosphate kinase ; Adenylate kinase 2, mitochondrial, N-terminally processed ; AK2 ; ADK2

Gene Name

AK2

Gene ID

204

UniProt

P54819

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human AK2 recombinant protein (Position: E161-I239) . Human AK2 shares 93.7% and 93% amino acid (aa) sequence identity with mouse and rat AK2, respectively.

Clonality

Polyclonal

Tissue Specificity

Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level) . .

Applications

IF, IHC, ICC, WB

Field of Research

Apoptosis, Cancer, Cell Biology, Cell Death, Energy Metabolism, Energy Transfer Pathways, Intracellular, Kinases, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis. .

References & Citations

1. Bruns GA, Regina VM (1977) . Adenylate kinase 2, a mitochondrial enzyme. Biochem. Genet. 15 (5–6) : 477–86. 2. Köhler C, Gahm A, Noma T, et al. (1999) . Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis. FEBS Lett. 447 (1) : 10–2.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26478 MW

Observed Molecular Weight

26 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Adenylate kinase 2, mitochondrial

Subcellular Location

Mitochondrion intermembrane space .

Protein Name

Adenylate kinase 2, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.