Anti-Apolipoprotein B/APOB Antibody Picoband®

About this product

Boster Bio Anti-Apolipoprotein B/APOB Antibody Picoband® catalog # PB10042. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10042

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Apolipoprotein B (ApoB) is a protein that in humans is encoded by the APOB gene. This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.

Synonyms

Apolipoprotein B-100; Apo B-100; Apolipoprotein B-48; Apo B-48; APOB

Gene Name

APOB

Gene ID

338

UniProt

P04114

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human Apolipoprotein B recombinant protein (Position: Q246-N450) . Human Apolipoprotein B shares 82.4% and 80.5% amino acid (aa) sequence identity with mouse and rat Apolipoprotein B, respectively.

Clonality

Polyclonal

Tissue Specificity

Widely expressed (at protein level) . Specifically expressed in the CNS. .

Applications

IHC, WB

Field of Research

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Cholesterol Metabolism, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100) . Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

References & Citations

1. Jacobson TA (2011) . Opening a new lipid apo-thecary: incorporating apolipoproteins as potential risk factors and treatment targets to reduce cardiovascular risk. Mayo Clinic Proceedings 86 (8) : 762–780. 2. Lim JS, Lee DH, Park JY, Jin SH, Jacobs DR (2011) . Reliability of low-absorbance lipoprotein cholesterol, non-high-absorbance lipoprotein cholesterol, and apolipoprotein B measurement.Journal of Clinical Lipidology 5 (4) : 264–272.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

515605 MW

Observed Molecular Weight

250 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Apolipoprotein B-100

Subcellular Location

Cytoplasm . Secreted .

Protein Name

Apolipoprotein B-100

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.