Anti-ARHGEF1 Antibody Picoband®

About this product

Boster Bio Anti-ARHGEF1 Antibody Picoband® catalog # PB10045. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10045

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Rho guanine nucleotide exchange factor 1 is a protein that in humans is encoded by the ARHGEF1 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Synonyms

Rho guanine nucleotide exchange factor 1;115 kDa guanine nucleotide exchange factor; p115-RhoGEF; p115RhoGEF; Sub1.5; ARHGEF1

Gene Name

ARHGEF1

Gene ID

9138

UniProt

Q92888

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ARHGEF1, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Signal Transduction, Signaling Pathway, G Protein Signaling, Regulators, Small G Proteins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation. .

References & Citations

1. Aasheim HC, Pedeutour F, Smeland EB (May 1997) . Characterization, expression and chromosomal localization of a human gene homologous to the mouse Lsc oncogene, with strongest expression in hematopoetic tissues. Oncogene 14 (14) : 1747–52. 2. Hart MJ, Sharma S, elMasry N, Qiu RG, McCabe P, Polakis P, Bollag G (Nov 1996) . Identification of a novel guanine nucleotide exchange factor for the Rho GTPase. J Biol Chem 271 (41) : 25452–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

102435 MW

Observed Molecular Weight

120 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Rho guanine nucleotide exchange factor 1

Subcellular Location

Cytoplasm . Membrane . Translocated to the membrane by activated GNA13 or LPA stimulation.

Protein Name

Rho guanine nucleotide exchange factor 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.