Anti-ACCN1/ASIC2 Antibody Picoband®

About this product

Boster Bio Anti-ACCN1/ASIC2 Antibody Picoband® catalog # PB10046. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10046

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Amiloride-sensitive cation channel 1, neuronal, also known as ASIC2, is a protein that in humans is encoded by the ACCN1 gene. This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified.

Synonyms

Acid-sensing ion channel 2; ASIC2; Amiloride-sensitive brain sodium channel; Amiloride-sensitive cation channel 1, neuronal; Amiloride-sensitive cation channel neuronal 1; Brain sodium channel 1; BNC1; BNaC1; Mammalian degenerin homolog; ASIC2; ACCN, ACCN1, BNAC1, MDEG

Gene Name

ASIC2

Gene ID

40

UniProt

Q16515

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ACCN1, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary. .

Applications

WB

Field of Research

Neuroscience, Neurotransmission, Receptors / Channels

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li (+) and K (+) . Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.

References & Citations

1. Vila-Carriles WH, Kovacs GG, Jovov B, Zhou ZH, Pahwa AK, Colby G, Esimai O, Gillespie GY, Mapstone TB, Markert JM, Fuller CM, Bubien JK, Benos DJ (Jul 2006) . Surface expression of ASIC2 inhibits the amiloride-sensitive current and migration of glioma cells. The Journal of Biological Chemistry 281 (28) : 19220–32. 2. Waldmann R, Voilley N, Mattéï MG, Lazdunski M (Oct 1996) . The human degenerin MDEG, an amiloride-sensitive neuronal cation channel, is localized on chromosome 17q11.2-17q12 close to the microsatellite D17S798.Genomics 37 (2) : 269–70.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

57709 MW

Observed Molecular Weight

65 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Acid-sensing ion channel 2

Subcellular Location

Cell membrane ; Multi-pass membrane protein . Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes. .

Protein Name

Acid-sensing ion channel 2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.