Anti-CD40 Antibody Picoband®

About this product

Boster Bio Anti-CD40 Antibody Picoband® catalog # PB10052. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10052

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.

Synonyms

Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CD40; Cd40; Tnfrsf5

Gene Name

Cd40

Gene ID

21939

UniProt

P27512

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived mouse CD40/TNFRSF5 recombinant protein (Position: L20-R193) . Mouse CD40/TNFRSF5 shares 60.7% amino acid (aa) sequence identity with human CD40/TNFRSF5.

Clonality

Polyclonal

Tissue Specificity

B-cells and in primary carcinomas.

Applications

ELISA, Flow Cytometry, IHC, ICC, WB

Field of Research

Adaptive Immunity, Atherosclerosis, B Cells, Cardiovascular, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. .

References & Citations

1. Clark, E. A. : CD40: a cytokine receptor in search of a ligand. Tissue Antigens 35: 33-36, 1990. 2. Harding, S. A.; Sarma, J.; Josephs, D. H.; Cruden, N. L.; Din, J. N.; Twomey, P. J.; Fox, K. A. A.; Newby, D. E. : Upregulation of the CD40/CD40 ligand dyad and platelet-monocyte aggregation in cigarette smokers. Circulation 109: 1926-1929, 2004. 3. Kato, K.; Cantwell, M. J.; Sharma, S.; Kipps, T. J. : Gene transfer of CD40-ligand induces autologous immune recognition of chronic lymphocytic leukemia B cells. J. Clin. Invest. 101: 1133-1141, 1998. 4. Stamenkovic, I.; Clark, E. A.; Seed, B. : A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas. EMBO J. 8: 1403-1410, 1989. 5. van Kooten, C.; Banchereau, J. : CD40-CD40 ligand. J. Leuko. Biol. 67: 2-17, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32093 MW

Observed Molecular Weight

40 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 5

Subcellular Location

Isoform I: Cell membrane; Single-pass type I membrane protein.

Protein Name

Tumor necrosis factor receptor superfamily member 5

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.