Anti-Neutrophil Elastase/ELANE Antibody Picoband®

About this product

Boster Bio Anti-Neutrophil Elastase/ELANE Antibody Picoband® catalog # PB10058. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10058

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN) . This gene is present in a gene cluster on chromosome 19.

Synonyms

Neutrophil elastase;3.4.21.37; Elastase-2; Leukocyte elastase; Elane; Ela2

Gene Name

Elane

Gene ID

50701

UniProt

Q3UP87

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived mouse Elastase/ELANE/ELA2 recombinant protein (Position: S27-N265) . Mouse Elastase/ELANE/ELA2 shares 92% amino acid (aa) sequence identity with rat Elastase/ELANE/ELA2.

Clonality

Polyclonal

Tissue Specificity

Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Applications

ELISA, IHC, WB

Field of Research

Calcium Signaling, Cancer, Complement, Cytoskeleton/ECM, Defensin, ECM Enzymes, ECM Proteins, Extracellular Matrix, Human Protein, Immunology, Innate Immunity, Invasion/Microenvironment, Macrophage/Inflammation, Microbiology, NK Cells, Protein, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (By similarity) . .

References & Citations

1. Ancliff, P. J., Gale, R. E., Liesner, R., Hann, I. M., Linch, D. C.Mutations in the ELA2 gene encoding neutrophil elastase are present in most patients with sporadic severe congenital neutropenia but only in some patients with the familial form of the disease. Blood 98: 2645-2650, 2001. 2. Bristow, C. L., Fiscus, S. A., Flood, P. M., Arnold, R. R. Inhibition of HIV-1 by modification of a host membrane protease. Int. Immun. 7: 239-249, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

29 kDa

Observed Molecular Weight

29 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Neutrophil elastase

Subcellular Location

Nucleus.

Protein Name

Neutrophil elastase

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.