Anti-FHL3 Antibody Picoband®

About this product

Boster Bio Anti-FHL3 Antibody Picoband® catalog # PB10064. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10064

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Four and a half LIM domains protein 3 is a protein that in humans is encoded by the FHL3 gene. The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Four and a half LIM domains protein 3; FHL-3; Skeletal muscle LIM-protein 2; SLIM-2; FHL3; SLIM2

Gene Name

FHL3

Gene ID

2275

UniProt

Q13643

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human FHL3, identical to the related mouse sequence.

Clonality

Polyclonal

Tissue Specificity

Expressed only in skeletal muscle.

Applications

WB

Field of Research

Cell Biology, Cell Cycle, Cell Differentiation, Cell Type Markers, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Tags & Cell Markers, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) . Activity requires the presence of KLB. .

References & Citations

1. Entrez Gene: FHL3 four and a half LIM domains 3. 2. Lee SM, Tsui SK, Chan KK, Kotaka M, Li HY, Chim SS, Waye MM, Fung KP, Lee CY (May 1999) . Chromosomal mapping of a skeletal muscle specific LIM-only protein FHL3 to the distal end of the short arm of human chromosome 1. Somat Cell Mol Genet 24 (3) : 197–202. 3. Morgan MJ, Madgwick AJ (October 1996) . Slim defines a novel family of LIM-proteins expressed in skeletal muscle. Biochem Biophys Res Commun 225 (2) : 632–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

31192 MW

Observed Molecular Weight

31 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Four and a half LIM domains protein 3

Subcellular Location

Secreted .

Protein Name

Four and a half LIM domains protein 3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.