Anti-GNB1 Antibody Picoband®

About this product

Boster Bio Anti-GNB1 Antibody Picoband® catalog # PB10067. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10067

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Guanine nucleotide-binding protein G (I) /G (S) /G (T) subunit beta-1 is a protein that in humans is encoded by the GNB1 gene. Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals.

Synonyms

Guanine nucleotide-binding protein G (I) /G (S) /G (T) subunit beta-1; Transducin beta chain 1; GNB1

Gene Name

GNB1

Gene ID

2782

UniProt

P62873

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human GNB1, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine.

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Cancer, Cancer Metabolism, Energy Transfer Pathways, G Protein Signaling, Heterotrimeric G Proteins, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.

References & Citations

1. Codina J, Stengel D, Woo SL, Birnbaumer L (1986) . Beta-subunits of the human liver Gs/Gi signal-transducing proteins and those of bovine retinal rod cell transducin are identical. FEBS Lett. 207 (2) : 187–92. 2. Yan K, Kalyanaraman V, Gautam N (March 1996) . Differential ability to form the G protein betagamma complex among members of the beta and gamma subunit families. J. Biol. Chem. 271 (12) : 7141–6.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

37377 MW

Observed Molecular Weight

37 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Guanine nucleotide-binding protein G (I) /G (S) /G (T) subunit beta-1

Subcellular Location

Cytoplasm, cell cortex . Cytoplasm, myofibril, sarcomere, Z line . Under the plasma membrane. After PMA stimulation, GLRX3 and PRKCQ/PKC-theta translocate to a more extended submembrane area. In the Z line, found associated with CSRP3 (By similarity) . .

Protein Name

Guanine nucleotide-binding protein G (I) /G (S) /G (T) subunit beta-1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.