Anti-HLTF Antibody Picoband®

About this product

Boster Bio Anti-HLTF Antibody Picoband® catalog # PB10070. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10070

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Helicase-like transcription factor is an enzyme that in humans is encoded by the HLTF gene. This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein.

Synonyms

Helicase-like transcription factor;3.6.4.-;6.3.2.-; DNA-binding protein/plasminogen activator inhibitor 1 regulator; HIP116; RING finger protein 80; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; Sucrose nonfermenting protein 2-like 3; HLTF; HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1

Gene Name

HLTF

Gene ID

6596

UniProt

Q14527

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human HLTF recombinant protein (Position: S911-L1009) . Human HLTF shares 92.9% amino acid (aa) sequence identity with mouse HLTF.

Clonality

Polyclonal

Tissue Specificity

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. .

Applications

IF, ICC, WB

Field of Research

Cancer, Cancer Susceptibility, Cell Biology,2339, Chromatin Remodeling, Epigenetics and Nuclear Signaling, Oncoproteins/Suppressors, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ring Finger E3 Ligase, Transcription, Tumor Suppressors, Ubiquitin E3 Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity) . These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind ly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA. .

References & Citations

1. Lin Y, Sheridan PL, Jones KA, Evans GA (Nov 1995) . The HIP116 SNF2/SW12-related transcription factor gene (SNF2L3) is located on human chromosome 3q25.1-q26.1.Genomics 27 (2) : 381–2. 2. Unk I, Hajdú I, Blastyák A, Haracska L (Jan 2010) . Role of yeast Rad5 and its human orthologs, HLTF and SHPRH in DNA damage tolerance. DNA Repair (Amst) 9 (3) : 257–67.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

113929 MW

Observed Molecular Weight

114 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Helicase-like transcription factor

Subcellular Location

Cytoplasm . Nucleus . Nucleus, nucleolus . Nucleus, nucleoplasm . Nuclear localization is stimulated by progesterone. .

Protein Name

Helicase-like transcription factor

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.