Anti-HPRT/HPRT1 Antibody Picoband®

About this product

Boster Bio Anti-HPRT/HPRT1 Antibody Picoband® catalog # PB10071. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10071

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Synonyms

Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase;2.4.2.8; HPRT1; HPRT

Gene Name

HPRT1

Gene ID

3251

UniProt

P00492

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human HPRT recombinant protein (Position: A2-S110) . Human HPRT shares 98.1% and 96.3% amino acid (aa) sequence identity with mouse and rat HPRT, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. .

Applications

WB

Field of Research

Cell Biology, DNA/Nucleotides, DNA/RNA, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.

References & Citations

1. Ansari MY, Dikhit MR, Sahoo GC, Das P (Apr 2012) . Comparative modeling of HGPRT enzyme of L. donovani and binding affinities of different analogs of GMP. International Journal of Biological Macromolecules50 (3) : 637–49. 2. Finette BA, Kendall H, Vacek PM (Aug 2002) . Mutational spectral analysis at the HPRT locus in healthy children. Mutation Research 505 (1-2) : 27–41.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

24579 MW

Observed Molecular Weight

25 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Hypoxanthine-guanine phosphoribosyltransferase

Subcellular Location

Cytoplasm.

Protein Name

Hypoxanthine-guanine phosphoribosyltransferase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.