Anti-LDHA Antibody Picoband®

About this product

Boster Bio Anti-LDHA Antibody Picoband® catalog # PB10075. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10075

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lactate dehydrogenase A, also known as LDHA, is an enzyme which in humans is encoded by the LDHA gene. The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Synonyms

L-lactate dehydrogenase A chain; LDH-A;1.1.1.27; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59; LDHA; PIG19

Gene Name

LDHA

Gene ID

3939

UniProt

P00338

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human LDHA recombinant protein (Position: A2-R106) . Human LDHA shares 94.3% amino acid (aa) sequence identity with both mouse and rat LDHA.

Clonality

Polyclonal

Tissue Specificity

Low levels in brain. Very weak expression found in heart and muscle. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Cancer Metabolism, Carbohydrate Metabolism, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Pathways and Processes, Signal Transduction, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity (By similarity) . Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH-dependent and independent manner, and inhibits ERK1/2 phosphorylation inly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction, and cell junction stabilization. Plays a role in integrin signaling via its interaction with ITGB1BP1; this prevents the interaction between ITGB1 and ITGB1BP1. Microtubule- associated protein that binds to phosphatidylinositol 4,5- bisphosphate (PIP2) -containing membranes in a GTP-bound RAP1- dependent manner. Plays an important role in the maintenance of the intracellular reactive oxygen species (ROS) homeostasis to prevent oxidative cellular damage. Regulates the homeostasis of intracellular ROS through an antioxidant pathway involving FOXO1 and SOD2. Facilitates the down-regulation of cyclin-D1 (CCND1) levels required for cell transition from proliferative growth to quiescence by preventing the accumulation of intracellular ROS through the modulation of FOXO1 and SOD2 levels. .

References & Citations

1. Chung FZ, Tsujibo H, Bhattacharyya U, Sharief FS, Li SS (November 1985) . Genomic organization of human lactate dehydrogenase-A gene. Biochem. J. 231 (3) : 537–41. 2. Gerdin AK (2010) . The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice. Acta Ophthalmologica 88: 925–7.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36689 MW

Observed Molecular Weight

37 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

L-lactate dehydrogenase A chain

Subcellular Location

Cytoplasm.

Protein Name

L-lactate dehydrogenase A chain

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.