Anti-MPP1 Antibody Picoband®

About this product

Boster Bio Anti-MPP1 Antibody Picoband® catalog # PB10078. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10078

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

55 kDa erythrocyte membrane protein is a protein that in humans is encoded by the MPP1 gene. This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

55 kDa erythrocyte membrane protein; p55; Membrane protein, palmitoylated 1; MPP1; DXS552E, EMP55

Gene Name

MPP1

Gene ID

4354

UniProt

Q00013

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MPP1, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. .

Applications

IF, ICC, WB

Field of Research

Adapters, Cardiovascular, Signal Transduction, Transmembrane

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity) . .

References & Citations

1. Bryant PJ, Woods DF (1992) . A major palmitoylated membrane protein of human erythrocytes shows homology to yeast guanylate kinase and to the product of a Drosophila tumor suppressor gene. Cell 68 (4) : 621–2. 2. Ruff P, Speicher DW, Husain-Chishti A (Sep 1991) .Molecular identification of a major palmitoylated erythrocyte membrane protein containing the src homology 3 motif. Proc Natl Acad Sci U S A 88 (15) : 6595–9.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

52296 MW

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

55 kDa erythrocyte membrane protein

Subcellular Location

Membrane; Lipid-anchor. Cell projection, stereocilium . Colocalizes with WHRN at stereocilium tip during hair cell development (By similarity) . Colocalizes with MPP5 in the retina, at the outer limiting membrane (OLM) . Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC) . Colocalizes with NF2 in non- myelin-forming Schwann cells. .

Protein Name

55 kDa erythrocyte membrane protein

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.