Anti-Proteasome 20S alpha 3/PSMA3 Antibody Picoband®

About this product

Boster Bio Anti-Proteasome 20S alpha 3/PSMA3 Antibody Picoband® catalog # PB10089. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10089

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proteasome subunit alpha type-3, also known as macropain subunit C8 and proteasome component C8, is a protein that in humans is encoded by the PSMA3 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified.

Synonyms

Proteasome subunit alpha type-3;3.4.25.1; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome component C8; PSMA3; HC8, PSC8

Gene Name

PSMA3

Gene ID

5684

UniProt

P25788

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human PSMA3, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Applications

Flow Cytometry, IHC, IHC-F, ICC, WB

Field of Research

Cell Biology, Proteasome, Proteasome / Ubiquitin, Proteolysis/Ubiquitin

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2. .

References & Citations

1. Coux O, Tanaka K, Goldberg AL (Nov 1996) . Structure and functions of the 20S and 26S proteasomes.Annu Rev Biochem 65: 801–47. 2. Tamura T, Lee DH, Osaka F, Fujiwara T, Shin S, Chung CH, Tanaka K, Ichihara A (Jun 1991) . Molecular cloning and sequence analysis of cDNAs for five major subunits of human proteasomes (multi-catalytic proteinase complexes) . Biochim Biophys Acta 1089 (1) : 95–102.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

28 kDa

Observed Molecular Weight

28 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Proteasome subunit alpha type-3

Subcellular Location

Cytoplasm. Nucleus.

Protein Name

Proteasome subunit alpha type-3

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.