- Catalog number
- PB10094
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Adipose differentiation-related protein, also known as perilipin 2 (PLIN2), ADRP or adipophilin, is a protein which in humans is encoded by the ADFP gene. The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
- Synonyms
- 40S ribosomal protein SA ;37 kDa laminin receptor precursor ;37LRP ;37/67 kDa laminin receptor ; LRP/LR ;67 kDa laminin receptor ;67LR ; Colon carcinoma laminin-binding protein; Laminin receptor 1 ; LamR ; Laminin-binding protein precursor p40 ; LBP/p40 ; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; RPSA ; LAMBR, LAMR1
- Gene Name
- RPSA
- Gene ID
- 3921
- UniProt
- P08865
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E. coli-derived human RPSA recombinant protein (Position: S2-S138) . Human RPSA shares 100% and 99.3% amino acid (aa) sequence identity with mouse and rat RPSA, respectively.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in a wide variety of actively proliferating cells and tumor tissues.
- Applications
- Flow Cytometry, IF, IHC, IHC-F, ICC, WB
- Field of Research
- Cell Biology, DNA/RNA, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ribosome, Translation
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA- precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. .
- References & Citations
- 1. Entrez Gene: RPSA ribosomal protein SA. 2. Jackers P, Minoletti F, Belotti D, Clausse N, Sozzi G, Sobel ME, Castronovo V (Sep 1996) . Isolation from a multigene family of the active human gene of the metastasis-associated multifunctional protein 37LRP/p40 at chromosome 3p21.3. Oncogene 13 (3) : 495–503. 3. Satoh K, Narumi K, Sakai T, Abe T, Kikuchi T, Matsushima K, Sindoh S, Motomiya M (Jul 1992) . Cloning of 67-kDa laminin receptor cDNA and gene expression in normal and malignant cell lines of the human lung. Cancer Lett 62 (3) : 199–203.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 32854 MW
- Observed Molecular Weight
- 40 kDa
- Specificity
- No cross reactivity with other proteins.
- Applications Notes
- 6
- Gene Name Synonym
- 40S ribosomal protein SA
- Subcellular Location
- Cell membrane. Cytoplasm. Nucleus . 67LR is found at the surface of the plasma membrane, with its C-terminal laminin- binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity) . Colocalizes with PPP1R16B in the cell membrane. .
- Protein Name
- 40S ribosomal protein SA
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
