Anti-PC4/SUB1 Antibody Picoband®

About this product

Boster Bio Anti-PC4/SUB1 Antibody Picoband® catalog # PB10098. Tested in IF, IHC WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10098

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA) -binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs.

Synonyms

Activated RNA polymerase II transcriptional coactivator p15; Positive cofactor 4; PC4; SUB1 homolog; p14; SUB1; PC4, RPO2TC1

Gene Name

SUB1

Gene ID

10923

UniProt

P53999

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PC4, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Expressed in leukocytes and plasma. .

Applications

IF, IHC, WB

Field of Research

Epigenetics and Nuclear Signaling, Pol II Transcription, Polymerase Associated Factors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA) . .

References & Citations

1. Entrez Gene SUB1: SUB1 homolog (S. cerevisiae) . 2. Kretzschmar M, Kaiser K, Lottspeich F, Meisterernst M (August 1994) . A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators. Cell 78 (3) : 525–34. 3. Ge H, Roeder RG (August 1994) . Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes. Cell 78 (3) : 513–23.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

14395 MW

Observed Molecular Weight

19 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Activated RNA polymerase II transcriptional coactivator p15

Subcellular Location

Nucleus.

Protein Name

Activated RNA polymerase II transcriptional coactivator p15

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.