Anti-Thrombopoietin/THPO Antibody Picoband®

About this product

Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # PB10102. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB10102

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.

Synonyms

Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; THPO; MGDF

Gene Name

THPO

Gene ID

7066

UniProt

P40225

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Thrombopoietin/TPO recombinant protein (Position: S22-R258) . Human Thrombopoietin/TPO shares 79.3% and 80.6% amino acid (aa) sequence identity with mouse and rat Thrombopoietin/TPO, respectively.

Clonality

Polyclonal

Tissue Specificity

Found in all tissues examined, most abundant in placenta and least abundant in brain and heart.

Applications

ELISA, WB

Field of Research

Adaptive Immunity, Domain Families, Epigenetics and Nuclear Signaling, Immunology, T Cells, Transcription, Zinc Finger

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

References & Citations

1. Entrez Gene: THPO thrombopoietin (myeloproliferative leukemia virus oncogene ligand, megakaryocyte growth and development factor) . 2. Kaushansky K (2006) . Lineage-specific hematopoietic growth factors. N. Engl. J. Med. 354 (19) : 2034–45.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

37823 MW

Observed Molecular Weight

45 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Thrombopoietin

Subcellular Location

Secreted.

Protein Name

Thrombopoietin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.