Anti-ACSL3 Antibody Picoband®

About this product

Boster Bio Anti-ACSL3 Antibody Picoband® catalog # A05945. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05945

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Long-chain-fatty-acid—CoA ligase 3 is an enzyme that in humans is encoded by the ACSL3 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.

Synonyms

Long-chain-fatty-acid--CoA ligase 3;6.2.1.3; Long-chain acyl-CoA synthetase 3; LACS 3; ACSL3; ACS3, FACL3, LACS3

Gene Name

ACSL3

Gene ID

2181

UniProt

O95573

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ACSL3 recombinant protein (Position: M12-I224) . Human ACSL3 shares 91.1% and 90.1% amino acid (aa) sequence identity with mouse and rat ACSL3, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level) . Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.

Applications

WB

Field of Research

Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity) . Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity) . Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) . .

References & Citations

1. Entrez Gene: ACSL3 acyl-CoA synthetase long-chain family member 3. 2. Bakken AM, Farstad M, Holmsen H (Feb 1991) .Identity between palmitoyl-CoA synthetase and arachidonoyl-CoA synthetase in human platelet?.The Biochemical Journal. 274 (Pt 1) (1) : 145–52. 3. Minekura H, Kang MJ, Inagaki Y, Suzuki H, Sato H, Fujino T, Yamamoto TT (Oct 2001) . Genomic organization and transcription units of the human acyl-CoA synthetase 3 gene. Gene. 278 (1-2) : 185–192.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

80420 MW

Observed Molecular Weight

80 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Long-chain-fatty-acid--CoA ligase 3

Subcellular Location

Mitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein . Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .

Protein Name

Long-chain-fatty-acid--CoA ligase 3

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.