Anti-Angiogenin/ANG Antibody Picoband®

About this product

Boster Bio Anti-Angiogenin/ANG Antibody Picoband® catalog # A00146. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00146

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Angiogenin (Ang), also known as ribonuclease 5, is a small 123 amino acid protein that in humans is encoded by the ANG gene. The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region.

Synonyms

Angiogenin;3.1.27.-; Ribonuclease 5; RNase 5; ANG; RNASE5

Gene Name

ANG

Gene ID

283

UniProt

P03950

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Angiogenin/ANG recombinant protein (Position: Q25-P147) . Human Angiogenin/ANG shares 77.5% amino acid (aa) sequence identity with mouse Angiogenin/ANG.

Clonality

Polyclonal

Tissue Specificity

Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. .

Applications

ELISA (Cap), IF, IHC, ICC, WB, Flow Cytometry

Field of Research

Actin Binding Proteins, Actin, etc., Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cytoskeleton, Cytoskeleton/ECM, DNA/RNA, Epigenetics and Nuclear Signaling, Hypoxia, Invasion/Microenvironment, Metabolism, Metabolism Processes, Microfilaments, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways and Processes, Response To Hypoxia, RNA Processing, Signal Transduction, Translation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs) . Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. .

References & Citations

1. Gao X, Xu Z (2008) . Mechanisms of action of angiogenin. Acta Biochimica et Biophysica Sinica. 40 (7) : 619–624. 2. Tello-Montoliu A; Patel J.V.; Lip G.Y.H. (2006) . Angiogenin: a review of the pathophysiology and potential clinical applications. Journal of Thrombosis and Haemostasis. 4 (9) : 1864–74. 3. Tsuji T, Sun Y, Kishimoto K, Olson K, Luo S, Hirukawa S, Hu G (2005) . Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation. Cancer Research (65) : 1352.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

16550 MW

Observed Molecular Weight

17 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Angiogenin

Subcellular Location

Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.

Protein Name

Angiogenin

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.