Anti-Calpastatin/CAST Antibody Picoband®

About this product

Boster Bio Anti-Calpastatin/CAST Antibody Picoband® catalog # A00337. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00337

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Calpastatin is a protein that in humans is encoded by the CAST gene. It is mapped to 5q15. The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.

Synonyms

Calpastatin; Calpain inhibitor; Sperm BS-17 component; CAST

Gene Name

CAST

Gene ID

831

UniProt

P20810

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Calpastatin, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by eleven amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in the islets of Langerhans (at protein level) .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Biochemicals, Chemical Type

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Specific inhibition of calpain (calcium-dependent cysteine protease) . Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

References & Citations

1. Averna M, De Tullio R, Capini P, Salamino F, Pontremoli S, Melloni E (Dec 2003) . Changes in calpastatin localization and expression during calpain activation: a new mechanism for the regulation of intracellular Ca (2+) -dependent proteolysis. Cell Mol Life Sci. 60 (12) : 2669–78. 2. Ma H, Yang HQ, Takano E, Lee WJ, Hatanaka M, Maki M (Sep 1993) . Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains. J Biochem. 113 (5) : 591–9. 3. Raynaud P, Jayat-Vignoles C, Laforet MP, Leveziel H, Amarger V (Apr 2005) . Four promoters expression of the calpastatin gene. Arch Biochem Biophys. 437 (1) : 69–77.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

76573 MW

Observed Molecular Weight

120 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Calpastatin

Subcellular Location

Nucleus.

Protein Name

Calpastatin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.