Anti-Cytochrome P450 17A1/CYP17A1 Antibody Picoband®

About this product

Boster Bio Anti-Cytochrome P450 17A1/CYP17A1 Antibody Picoband® catalog # A00615-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00615-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytochrome P450 17A1, also called steroid 17α-monooxygenase, is an enzyme of the hydroxylase type that in humans is encoded by the CYP17A1 gene on chromosome 10. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.

Synonyms

Steroid 17-alpha-hydroxylase/17,20 lyase;1.14.14.19 ;17-alpha-hydroxyprogesterone aldolase;4.1.2.30 ; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; Steroid 17-alpha-monooxygenase; CYP17A1; CYP17, S17AH

Gene Name

CYP17A1

Gene ID

1586

UniProt

P05093

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CYP17A1, different from the related mouse and rat sequences by ten amino acids.

Clonality

Polyclonal

Tissue Specificity

Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen. .

Applications

Flow Cytometry, IHC, WB

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Cytochromes, Endocrine Metabolism, Growth Factors, Growth Factors/Hormones, Hormone Biosynthesis, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. .

References & Citations

1. DeVore NM, Scott EE (February 2012) . Structures of cytochrome P450 17A1 with prostate cancer drugs abiraterone and TOK-001. Nature. 482 (7383) : 116–9. 2. Estrada DF, Laurence JS, Scott EE (February 2016) . Cytochrome P450 17A1 Interactions with the FMN Domain of Its Reductase as Characterized by NMR. The Journal of Biological Chemistry. 291 (8) : 3990–4003. 3. Petrunak EM, DeVore NM, Porubsky PR, Scott EE (November 2014) . Structures of human steroidogenic cytochrome P450 17A1 with substrates.The Journal of Biological Chemistry. 289 (47) : 32952–64.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

57371 MW

Observed Molecular Weight

57 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Steroid 17-alpha-hydroxylase/17,20 lyase

Subcellular Location

Membrane .

Protein Name

Steroid 17-alpha-hydroxylase/17,20 lyase

Isotype

Rabbit IgG

Contents

243