Anti-EPHX2 Antibody Picoband®

About this product

Boster Bio Anti-EPHX2 Antibody Picoband® catalog # A01999-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01999-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Soluble epoxide hydrolase (sEH) is a bifunctional enzyme that in humans is encoded by the EPHX2 gene. It is mapped to 8p21.2-p21.1. This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.

Synonyms

Bifunctional epoxide hydrolase 2; Cytosolic epoxide hydrolase 2; CEH;3.3.2.10; Epoxide hydratase; Soluble epoxide hydrolase; SEH; Lipid-phosphate phosphatase;3.1.3.76; EPHX2

Gene Name

EPHX2

Gene ID

2053

UniProt

P34913

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human EPHX2, different from the related mouse sequence by seven amino acids, and from the related rat sequence by eight amino acids.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. A high level expression is seen in secretory tissues.

Applications

Flow Cytometry, WB

Field of Research

Cancer Metabolism, Cardiovascular, Cell Biology, Cholesterol Metabolism, Drug Metabolism, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Signal Transduction, Vasculature

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate. .

References & Citations

1. Entrez Gene: Epoxide hydrolase 2, cytoplasmic. 2. Morisseau C, Hammock BD (2013) . Impact of soluble epoxide hydrolase and epoxyeicosanoids on human health.Annu. Rev. Pharmacol. Toxicol. 53: 37–58. 3. Lee CR, North KE, Bray MS, Fornage M, Seubert JM, Newman JW, Hammock BD, Couper DJ, Heiss G, Zeldin DC (May 2006) . Genetic variation in soluble epoxide hydrolase (EPHX2) and risk of coronary heart disease: The Atherosclerosis Risk in Communities (ARIC) study. Hum. Mol. Genet.15 (10) : 1640–9.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

62616 MW

Observed Molecular Weight

63 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Bifunctional epoxide hydrolase 2

Subcellular Location

Cytoplasm. Peroxisome.

Protein Name

Bifunctional epoxide hydrolase 2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.