Anti-Hexokinase II/HK2 Antibody Picoband®

About this product

Boster Bio Anti-Hexokinase II/HK2 Antibody Picoband® catalog # A01389. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01389

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hexokinase 2, also known as HK2, is an enzyme which in humans is encoded by the HK2 gene on chromosome 2. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Synonyms

Hexokinase-2;2.7.1.1; Hexokinase type II; HK II; Muscle form hexokinase; HK2

Gene Name

HK2

Gene ID

3099

UniProt

P52789

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Hexokinase II, different from the related mouse and rat sequences by four amino acids.

Clonality

Polyclonal

Tissue Specificity

Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.

Applications

Flow Cytometry, IHC, WB

Field of Research

Cancer, Cancer Metabolism, Carbohydrate Metabolism, Cardiovascular, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis (By similarity) . Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.

References & Citations

1. Entrez Gene: HK2 hexokinase 2. 2. Printz RL, Osawa H, Ardehali H, Koch S, Granner DK (Feb 1997) . Hexokinase II gene: structure, regulation and promoter organization. Biochemical Society Transactions. 25 (1) : 107–12. 3. Peng Q, Zhou J, Zhou Q, Pan F, Zhong D, Liang H (2009) . Silencing hexokinase II gene sensitizes human colon cancer cells to 5-fluorouracil. Hepato-Gastroenterology. 56 (90) : 355–60.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

102 kDa

Observed Molecular Weight

102 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Hexokinase-2

Subcellular Location

Mitochondrion outer membrane . Its hydrophobic N-terminal sequence may be involved in membrane binding. .

Protein Name

Hexokinase-2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.