Anti-HnRNP F/HNRNPF Antibody Picoband®

About this product

Boster Bio Anti-HnRNP F/HNRNPF Antibody Picoband® catalog # A05806. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05806

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Heterogeneous nuclear ribonucleoprotein F is a protein that in humans is encoded by the HNRNPF gene. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) . The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) . These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Synonyms

Heterogeneous nuclear ribonucleoprotein F; hnRNP F; Nucleolin-like protein mcs94-1; Heterogeneous nuclear ribonucleoprotein F, N-terminally processed; HNRNPF; HNRPF

Gene Name

HNRNPF

Gene ID

3185

UniProt

P52597

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human HnRNP F, different from the related mouse and rat sequences by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed ubiquitously.

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Chromatin Binding Proteins, DNA/RNA, DNA/RNA Binding, Epigenetics and Nuclear Signaling, RNA Processing, Splicing

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state. .

References & Citations

1. Entrez Gene: HNRPF heterogeneous nuclear ribonucleoprotein F. 2. Honore B, Rasmussen HH, Vorum H, Dejgaard K, Liu X, Gromov P, Madsen P, Gesser B, Tommerup N, Celis JE (Jan 1996) . Heterogeneous nuclear ribonucleoproteins H, H', and F are members of a ubiquitously expressed subfamily of related but distinct proteins encoded by genes mapping to different chromosomes. J Biol Chem. 270 (48) : 28780–9. 3. Matunis MJ, Xing J, Dreyfuss G (1994) . The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization.. Nucleic Acids Res. 22 (6) : 1059–67.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

45672 MW

Observed Molecular Weight

46 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Heterogeneous nuclear ribonucleoprotein F

Subcellular Location

Nucleus, nucleoplasm.

Protein Name

Heterogeneous nuclear ribonucleoprotein F

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.