Anti-Cytokeratin 14/KRT14 Antibody Picoband®

About this product

Boster Bio Anti-Cytokeratin 14/KRT14 Antibody Picoband® catalog # A01432. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01432

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytokeratin 14, also known as cytokeratin-14 (CK-14) or keratin-14 (KRT14), is a member of the type I keratin family of intermediate filament proteins. In humans it is encoded by the KRT14 gene. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

Synonyms

Keratin, type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14; KRT14

Gene Name

KRT14

Gene ID

3861

UniProt

P02533

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 14, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. .

Applications

Flow Cytometry, IHC, IHC-F, ICC, WB

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. .

References & Citations

1. Batta, K., Rugg, E. L., Wilson, N. J., West, N., Goodyear, H., Lane, E. B., Gratian, M., Dopping-Hepenstal, P., Moss, C., Eady, R. A. J. A keratin 14 'knockout' mutation in recessive epidermolysis bullosa simplex resulting in less severe disease. Brit. J. Derm. 143: 621-627, 2000. 2. Chan, Y., Anton-Lamprecht, I., Yu, Q.-C., Jackel, A., Zabel, B., Ernst, J.-P., Fuchs, E. A human keratin 14 'knockout': the absence of K14 leads to severe epidermolysis bullosa simplex and a function for an intermediate filament protein. Genes Dev. 8: 2574-2587, 1994. 3. Chen, H., Bonifas, J. M., Mstsumura, K., Ikeda, S., Leyden, W. A., Epstein, E. H. Keratin 14 gene mutations in patients with epidermolysis bullosa simplex. J. Invest. Derm. 105: 629-632, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

51561 MW

Observed Molecular Weight

60 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Keratin, type I cytoskeletal 14

Subcellular Location

Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.

Protein Name

Keratin, type I cytoskeletal 14

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.