Anti-ACSL5 Antibody Picoband®

About this product

Boster Bio Anti-ACSL5 Antibody Picoband® catalog # A05087-2. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05087-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Long-chain-fatty-acid—CoA ligase 5 is an enzyme that in humans is encoded by the ACSL5 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.

Synonyms

Long-chain-fatty-acid--CoA ligase 5;6.2.1.3; Long-chain acyl-CoA synthetase 5; LACS 5; ACSL5; ACS5, FACL5; UNQ633/PRO1250

Gene Name

ACSL5

Gene ID

51703

UniProt

Q9ULC5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ACSL5, different from the related mouse and rat sequences by six amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Applications

WB, Flow Cytometry

Field of Research

Cancer, Lipid Metabolism, Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity) . Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity) . It was suggested that it may also stimulate fatty acid oxidation (By similarity) . At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells. .

References & Citations

1. Entrez Gene: ACSL5 acyl-CoA synthetase long-chain family member 5. 2. Yamashita Y, Kumabe T, Cho YY, Watanabe M, Kawagishi J, Yoshimoto T, Fujino T, Kang MJ, Yamamoto TT (Dec 2000) . Fatty acid induced glioma cell growth is mediated by the acyl-CoA synthetase 5 gene located on chromosome 10q25.1-q25.2, a region frequently deleted in malignant gliomas. Oncogene. 19 (51) : 5919–25.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

75991 MW

Observed Molecular Weight

76 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Long-chain-fatty-acid--CoA ligase 5

Subcellular Location

Mitochondrion. Endoplasmic reticulum. Mitochondrion outer membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .

Protein Name

Long-chain-fatty-acid--CoA ligase 5

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.