Anti-Anterior Gradient 2/AGR2 Antibody Picoband®

About this product

Boster Bio Anti-Anterior Gradient 2/AGR2 Antibody Picoband® catalog # A02922-2. Tested in Flow Cytometry, IP, ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02922-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Anterior gradient protein 2 homolog (AGR-2), also known as secreted cement gland protein XAG-2 homolog, is a protein that in humans is encoded by the AGR2 gene. Anterior gradient homolog 2 was originally discovered in Xenopus laevis. In Xenopus AGR2 plays a role in cement gland differentiation, but in human cancer cell lines high levels of AGR2 correlate with downregulation of the p53 response, cell migration, and cell transformation. However, there have been other observations that AGR2 can repress growth and proliferation.

Synonyms

Anterior gradient protein 2 homolog; AG-2; hAG-2; HPC8; Secreted cement gland protein XAG-2 homolog; AGR2; AG2; UNQ515/PRO1030

Gene Name

AGR2

Gene ID

10551

UniProt

O95994

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Anterior Gradient 2 recombinant protein (Position: R21-L175) . Human Anterior Gradient 2 shares 93.5% amino acid (aa) sequence identity with mouse Anterior Gradient 2.

Clonality

Polyclonal

Tissue Specificity

Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER) -positive breast cancer cell lines. .

Applications

Flow Cytometry, IP, ICC/IF, IHC, WB

Field of Research

Cancer, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity) . Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. .

References & Citations

1. Aberger F, Weidinger G, Grunz H, Richter K (March 1998) . Anterior specification of embryonic ectoderm: the role of the Xenopus cement gland-specific gene XAG-2. Mech. Dev. 72 (1–2) : 115–30. 2. Bu H, Bormann S, Schäfer G, Horninger W, Massoner P, Neeb A, Lakshmanan VK, Maddalo D, Nestl A, Sültmann H, Cato AC, Klocker H (May 2011) . The anterior gradient 2 (AGR2) gene is overexpressed in prostate cancer and may be useful as a urine sediment marker for prostate cancer detection. Prostate. 71 (6) : 575–87. 3. Vanderlaag KE, Hudak S, Bald L, Fayadat-Dilman L, Sathe M, Grein J, Janatpour MJ (2010) . Anterior gradient-2 plays a critical role in breast cancer cell growth and survival by modulating cyclin D1, estrogen receptor-alpha and survivin. Breast Cancer Res. 12 (3) : R32.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

19979 MW

Observed Molecular Weight

20 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Anterior gradient protein 2 homolog

Subcellular Location

Secreted . Endoplasmic reticulum .

Protein Name

Anterior gradient protein 2 homolog

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.