Anti-Iba1/AIF1 Antibody Picoband®

About this product

Boster Bio Anti-Iba1/AIF1 Antibody Picoband® catalog # A01394. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Pig. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01394

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Allograft inflammatory factor 1 (AIF-1), also known as ionized calcium-binding adapter molecule 1 (IBA1), is a protein that in humans is encoded by the AIF1 gene. This gene encodes a protein that binds actin and calcium. And this gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.

Synonyms

Allograft inflammatory factor 1; AIF-1; Ionized calcium-binding adapter molecule 1; Protein G1; AIF1; G1, IBA1

Gene Name

AIF1

Gene ID

199

UniProt

P55008

Host

Rabbit

Reactivity

Human, Mouse, Rat, Pig

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Iba1, different from the related mouse sequence by seven amino acids, and from the related rat sequence by six amino acids.

Clonality

Polyclonal

Tissue Specificity

Detected in T-lymphocytes and peripheral blood mononuclear cells. .

Applications

IHC, WB

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. .

References & Citations

1. Entrez Gene: AIF1 allograft inflammatory factor 1. 2. Utans U, Arceci RJ, Yamashita Y, Russell ME (June 1995) . Cloning and characterization of allograft inflammatory factor-1: a novel macrophage factor identified in rat cardiac allografts with chronic rejection. J. Clin. Invest. 95 (6) : 2954–62. 3. Tian Y, Jain S, Kelemen SE, Autieri MV (February 2009) . AIF-1 expression regulates endothelial cell activation, signal transduction, and vasculogenesis. Am. J. Physiol., Cell Physiol. 296 (2) : C256–66.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

16703 MW

Observed Molecular Weight

17 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Allograft inflammatory factor 1

Subcellular Location

Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

Protein Name

Allograft inflammatory factor 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.