Anti-Bcl-XL/BCL2L1 Antibody Picoband®

About this product

Boster Bio Anti-Bcl-XL/BCL2L1 Antibody Picoband® catalog # A00181. Tested in Flow Cytometry, IP, ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00181

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Bcl-2-like protein 1, also known as Bcl-XL, is a protein that in humans is encoded by the BCL2L1 gene. The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.

Synonyms

Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; BCL2L1; BCL2L, BCLX

Gene Name

BCL2L1

Gene ID

598

UniProt

Q07817

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Bcl-XL recombinant protein (Position: M1-T219) . Human Bcl-XL shares 97.9% amino acid (aa) sequence identity with both mouse and rat Bcl-XL.

Clonality

Polyclonal

Tissue Specificity

Bcl-X (S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X (L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Applications

Flow Cytometry, IP, ICC/IF, IHC, WB

Field of Research

Apoptosis, Apoptotic Markers, Cancer, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage- dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.

References & Citations

1. Entrez Gene: BCL2L1 BCL2-like 1. 2. Boise LH, González-García M, Postema CE, Ding L, Lindsten T, Turka LA, Mao X, Nuñez G, Thompson CB (Aug 1993) . bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death. Cell 74 (4) : 597–608.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26049 MW

Observed Molecular Weight

30 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Bcl-2-like protein 1

Subcellular Location

Isoform Bcl-X (L) : Mitochondrion inner membrane . Mitochondrion outer membrane . Mitochondrion matrix . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus membrane ; Single-pass membrane protein ; Cytoplasmic side . After neuronal stimulation, translocates from cytosol to synaptic vesicle and mitochondrion membrane in a calmodulin-dependent manner (By similarity) . Localizes to the centrosome when phosphorylated at Ser-49. .

Protein Name

Bcl-2-like protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.