Anti-Alpha Defensin 1/DEFA1 Antibody Picoband®

About this product

Boster Bio Anti-Alpha Defensin 1/DEFA1 Antibody Picoband® catalog # A10546. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A10546

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Defensin, alpha 1, also known as human alpha defensin 1, human neutrophil peptide 1 (HNP-1) or neutrophil defensin 1 is a human protein that is encoded by the DEFA1 gene. Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.

Synonyms

Neutrophil defensin 1; Defensin, alpha 1; HNP-1; HP-1; HP1; HP 1-56; Neutrophil defensin 2; HNP-2; HP-2; HP2; DEFA1; DEF1, DEFA2, MRS; DEFA1B

Gene Name

DEFA1

Gene ID

1667

UniProt

P59665

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Alpha Defensin 1.

Clonality

Polyclonal

Tissue Specificity

Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Applications

WB

Field of Research

Defensin, Human Protein, Immunology, Innate Immunity, Macrophage/Inflammation, Microbiology, Protein, Secreted Molecules

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. .

References & Citations

1. Entrez Gene: DEFA1 defensin, alpha 1. 2. Aldred PM, Hollox EJ, Armour JA (Jul 2005) . Copy number polymorphism and expression level variation of the human alpha-defensin genes DEFA1 and DEFA3.Hum Mol Genet. 14 (14) : 2045–52. 3. Feng Y, Gutekunst CA, Eberhart DE, Yi H, Warren ST, Hersch SM (Mar 1997) . Fragile X mental retardation protein: nucleocytoplasmic shuttling and association with somatodendritic ribosomes. J Neurosci. 17 (5) : 1539–47.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

10201 MW

Observed Molecular Weight

19 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Neutrophil defensin 1

Subcellular Location

Secreted.

Protein Name

Neutrophil defensin 1

Isotype

Rabbit IgG

Contents

243