Anti-Leptin Receptor/LEPR Antibody

About this product

Boster Bio Anti-Leptin Receptor/LEPR Antibody Picoband® catalog # A00350. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00350

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Leptin receptor, also known as LEP-R or OB-R is a protein that in humans is encoded by the LEPR gene. The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Synonyms

Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295; LEPR; DB, OBR

Gene Name

LEPR

Gene ID

3953

UniProt

P48357

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Leptin Receptor, different from the related mouse sequence by nine amino acids, and from the related rat sequence by eight amino acids.

Clonality

Polyclonal

Tissue Specificity

Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.

Applications

ELISA, IHC

Field of Research

Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction, Structures

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor for obesity factor (leptin) . On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity) . .

References & Citations

1. Entrez Gene: LEPR leptin receptor. 2. Tartaglia LA, Dembski M, Weng X, Deng N, Culpepper J, Devos R, Richards GJ, Campfield LA, Clark FT, Deeds J, Muir C, Sanker S, Moriarty A, Moore KJ, Smutko JS, Mays GG, Wool EA, Monroe CA, Tepper RI (Feb 1996) . Identification and expression cloning of a leptin receptor, OB-R. Cell. 83 (7) : 1263–71. 3. Winick JD, Stoffel M, Friedman JM (Feb 1997) . Identification of microsatellite markers linked to the human leptin receptor gene on chromosome 1.Genomics. 36 (1) : 221–2.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

132494 MW

Observed Molecular Weight

27 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Leptin receptor

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Name

Leptin receptor

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.