Anti-Nephrin/NPHS1 Antibody Picoband®

About this product

Boster Bio Anti-Nephrin/NPHS1 Antibody Picoband® catalog # A01991. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01991

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NPHS1, also called Nephrin, is mapped to 19q13.12. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. It gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. A defect in the gene for nephrin, NPHS1, is associated with congenital nephrotic syndrome of the Finnish type and causes massive amounts of protein to be leaked into the urine, orproteinuria. NPHS1 is also required for cardiovascular development.

Synonyms

Nephrin; Renal glomerulus-specific cell adhesion receptor; NPHS1; NPHN

Gene Name

NPHS1

Gene ID

4868

UniProt

O60500

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Nephrin recombinant protein (Position: V1084-V1241) . Human Nephrin shares 79.9% and 75.3% amino acid (aa) sequence identity with mouse and rat Nephrin, respectively.

Clonality

Polyclonal

Tissue Specificity

Specifically expressed in podocytes of kidney glomeruli.

Applications

IHC, WB

Field of Research

Cell Adhesion, Cell Adhesion Molecules, Cytoskeleton/ECM, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity) . .

References & Citations

1. Wagner, N; Morrison, H; Pagnotta, S; Michiels, JF; Schwab, Y; Tryggvason, K; Schedl, A; Wagner, KD (2011-03-29) . The podocyte protein nephrin is required for cardiac vessel formation.. Human Molecular Genetics 20 (11) : 2182–94. 2. Wartiovaara, J., Ofverstedt, L.-G., Khoshnoodi, J., Zhang, J., Makela, E., Sandin, S., Ruotsalainen, V., Cheng, R. H., Jalanko, H., Skoglund, U., Tryggvason, K. Nephrin strands contribute to a porous slit diaphragm scaffold as revealed by electron tomography. J. Clin. Invest. 114: 1475-1483, 2004.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

134742 MW

Observed Molecular Weight

185 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Nephrin

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. .

Protein Name

Nephrin

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.