Anti-Periplakin/PPL Antibody Picoband®

About this product

Boster Bio Anti-Periplakin/PPL Antibody Picoband® catalog # A02098-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02098-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Periplakin is a protein that in humans is encoded by the PPL gene. The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.

Synonyms

Periplakin;190 kDa paraneoplastic pemphigus antigen;195 kDa cornified envelope precursor protein; PPL; KIAA0568

Gene Name

PPL

Gene ID

5493

UniProt

O60437

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Periplakin, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Expressed in stratified squamous epithelia and in some other epithelia. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling. .

References & Citations

1. Entrez Gene: PPL periplakin. 2. Aho S, McLean WH, Li K, Uitto J (Jun 1998) . cDNA cloning, mRNA expression, and chromosomal mapping of human and mouse periplakin genes. Genomics. 48 (2) : 242–7. 3. Kazerounian, Shideh; Uitto Jouni; Aho Sirpa (Oct 2002) . Unique role for the periplakin tail in intermediate filament association: specific binding to keratin 8 and vimentin. Exp. Dermatol. Denmark. 11 (5) : 428–38.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

204747 MW

Observed Molecular Weight

202 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Periplakin

Subcellular Location

Cell junction, desmosome . Cytoplasm, cytoskeleton . Cell membrane . Nucleus . Mitochondrion . Associated with desmosomes and intermediate filaments.

Protein Name

Periplakin

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.