Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody Picoband®

About this product

Boster Bio Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody Picoband® catalog # A00699-1. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00699-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CAMP-dependent protein kinase type I-alpha regulatory subunit is an enzyme that in humans is encoded by the PRKAR1A gene. This protein encoded by this gene was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC) . This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40) . Several alternatively spliced transcript variants encoding two different isoforms have been observed.

Synonyms

CAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed; PRKAR1A; PKR1, PRKAR1, TSE1

Gene Name

PRKAR1A

Gene ID

5573

UniProt

P10644

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PRKAR1A recombinant protein (Position: E2-E81) . Human PRKAR1A shares 89.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PRKAR1A, respectively.

Clonality

Polyclonal

Tissue Specificity

Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Applications

IHC, WB

Field of Research

Cancer, Cell Cycle, DNA/RNA, Epigenetics and Nuclear Signaling, Kinases/Phosphatases, Nuclear Import/Export, Protein Trafficking, RNA Processing, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. .

References & Citations

1. Entrez Gene: PRKAR1A protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) . 2. Scambler P, Oyen O, Wainwright B, Farrall M, Law HY, Estivill X, Sandberg M, Williamson R, Jahnsen T (December 1987) . Exclusion of catalytic and regulatory subunits of cAMP-dependent protein kinase as candidate genes for the defect causing cystic fibrosis. Am J Hum Genet. 41 (5) : 925–32.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42982 MW

Observed Molecular Weight

48 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/mL, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/mL, Human, Rat<br>

Gene Name Synonym

CAMP-dependent protein kinase type I-alpha regulatory subunit

Subcellular Location

Cell membrane .

Protein Name

CAMP-dependent protein kinase type I-alpha regulatory subunit

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.