- Catalog number
- A00650-1
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Telomeric repeat-binding factor 2, also known as TERF2, TRF2, or TRBF2, is encoded in humans by the TERF2 gene. This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic.
- Synonyms
- Telomeric repeat-binding factor 2; TTAGGG repeat-binding factor 2; Telomeric DNA-binding protein; TERF2; TRBF2, TRF2
- Gene Name
- TERF2
- Gene ID
- 7014
- UniProt
- Q15554
- Host
- Rabbit
- Reactivity
- Human, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- E.coli-derived human TRF2 recombinant protein (Position: A81-K287) . Human TRF2 shares 95.7% amino acid (aa) sequence identity with mouse TRF2.
- Clonality
- Polyclonal
- Tissue Specificity
- Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
- Applications
- IHC, WB
- Field of Research
- Cancer, Nuclear Import/Export, Protein Trafficking, Signal Transduction
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single- stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology- ed repair (HDR), which can affect telomere length. .
- References & Citations
- 1. Entrez Gene: TERF2 telomeric repeat binding factor 2. 2. Opresko PL, von Kobbe C, Laine JP, Harrigan J, Hickson ID, Bohr VA (October 2002) . Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases. J. Biol. Chem. 277 (43) : 41110–9. 3. Sakaguchi, A. Y.; Padalecki, S. S.; Mattern, V.; Rodriguez, A.; Leach, R. J.; McGill, J. R.; Chavez, M.; Giambernardi, T. A. (1998-05-01) . Chromosomal sublocalization of the transcribed human telomere repeat binding factor 2 gene and comparative mapping in the mouse. Somatic Cell and Molecular Genetics. 24 (3) : 157–163.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 59594 MW
- Observed Molecular Weight
- 75 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Telomeric repeat-binding factor 2
- Subcellular Location
- Nucleus . Chromosome, telomere . Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
- Protein Name
- Telomeric repeat-binding factor 2
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
