Anti-APRT Antibody Picoband®

About this product

Boster Bio Anti-APRT Antibody Picoband® catalog # A02721. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02721

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Adenine phosphoribosyltransferase (APRTase) is an enzyme encoded by the APRT gene, found in humans on chromosome 16. It belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP) . It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Adenine phosphoribosyltransferase; APRT;2.4.2.7; APRT

Gene Name

APRT

Gene ID

353

UniProt

P07741

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human APRT, different from the related mouse sequence by ten amino acids, and from the related rat sequence by nine amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole.

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Cancer, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

References & Citations

1. Silva CH, Silva M, Iulek J, Thiemann OH (Jun 2008) . Structural complexes of human adenine phosphoribosyltransferase reveal novel features of the APRT catalytic mechanism. Journal of Biomolecular Structure & Dynamics. 25 (6) : 589–97. 2. Sarver AE, Wang CC (Oct 2002) . The adenine phosphoribosyltransferase from Giardia lamblia has a unique reaction mechanism and unusual substrate binding properties. The Journal of Biological Chemistry. 277 (42) : 39973–80. 3. Valaperta R, Rizzo V, Lombardi F, Verdelli C, Piccoli M, Ghiroldi A, Creo P, Colombo A, Valisi M, Margiotta E, Panella R, Costa E (1 July 2014) . Adenine phosphoribosyltransferase (APRT) deficiency: identification of a novel nonsense mutation. BMC Nephrology. 15: 102.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

19608 MW

Observed Molecular Weight

20 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Adenine phosphoribosyltransferase

Subcellular Location

Cytoplasm.

Protein Name

Adenine phosphoribosyltransferase

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.