Anti-Diazepam Binding Inhibitor/DBI Antibody Picoband®

About this product

Boster Bio Anti-Diazepam Binding Inhibitor/DBI Antibody Picoband® catalog # A01267. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01267

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Acyl-CoA-binding protein is a protein that in humans is encoded by the DBI gene. This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.

Synonyms

Acyl-CoA-binding protein; ACBP; Diazepam-binding inhibitor; DBI; Endozepine; EP; DBI

Gene Name

DBI

Gene ID

1622

UniProt

P07108

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human DBI recombinant protein (Position: S2-I87) . Human DBI shares 77.9% amino acid (aa) sequence identity with both mouse and rat DBI.

Clonality

Polyclonal

Tissue Specificity

Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart. .

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Apoptosis, Calcium Channels, Calcium Signaling, Cancer, Cardiovascular, Cell Biology, Cell Death, Cholesterol Metabolism, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.

References & Citations

1. Entrez Gene: DBI diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein) . 2. Mandrup S, Hummel R, Ravn S, et al. (1993) . Acyl-CoA-binding protein/diazepam-binding inhibitor gene and pseudogenes. A typical housekeeping gene family. J. Mol. Biol. 228 (3) : 1011–22. 3. Todd S, Naylor SL (Dec 1992) . New chromosomal mapping assignments for argininosuccinate synthetase pseudogene 1, interferon-beta 3 gene, and the diazepam binding inhibitor gene. Somat Cell Mol Genet. 18 (4) : 381–5.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

10044 MW

Observed Molecular Weight

10 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Acyl-CoA-binding protein

Subcellular Location

Endoplasmic reticulum . Golgi apparatus . Golgi localization is dependent on ligand binding (PubMed:17953517) . .

Protein Name

Acyl-CoA-binding protein

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.