Anti-HnRNP L/HNRNPL Antibody Picoband®

About this product

Boster Bio Anti-HnRNP L/HNRNPL Antibody Picoband® catalog # A03769. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03769

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Heterogeneous nuclear ribonucleoprotein L is a protein that in humans is encoded by the HNRNPL gene. Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. HNRNPL is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. It is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Heterogeneous nuclear ribonucleoprotein L; hnRNP L; HNRNPL; HNRPL; P/OKcl.14

Gene Name

HNRNPL

Gene ID

3191

UniProt

P14866

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HnRNP L, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level) . Widely expressed. Weakly expressed in leukocytes and fetal thymus.

Applications

IF, IHC, ICC, WB

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, Nucleus, RNA Processing, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements (PubMed:11809897, PubMed:22570490, PubMed:24164894, PubMed:25623890, PubMed:26051023) . Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts (PubMed:2687284) . Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter (PubMed:11809897) . .

References & Citations

1. Entrez Gene: HNRPL heterogeneous nuclear ribonucleoprotein L. 2. Hahm B, Cho OH, Kim JE, Kim YK, Kim JH, Oh YL, Jang SK (April 1998) . Polypyrimidine tract-binding protein interacts with HnRNP L. FEBS Lett. 425 (3) : 401–6. 3. Pinol-Roma S, Swanson MS, Gall JG, Dreyfuss G (January 1990) . A novel heterogeneous nuclear RNP protein with a unique distribution on nascent transcripts. J Cell Biol. 109 (6 Pt 1) : 2575–87.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

64133 MW

Observed Molecular Weight

64 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Heterogeneous nuclear ribonucleoprotein L

Subcellular Location

Nucleus, nucleoplasm . Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. These granules are not identical with P bodies or stress granules. .

Protein Name

Heterogeneous nuclear ribonucleoprotein L

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.