Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband®

About this product

Boster Bio Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband® catalog # A04485. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04485

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interferon regulatory factor 9 is a protein that in humans is encoded by the IRF9 gene, previously known as ISGF3G. It is mapped to 14q1. IRF9 is a 48 kDa member of the IRF family of proteins. It is widely expressed, and serves as a component of the ISGF3 complex. In addition, IRF9 is an interferon-dependent, positive-acting transcription factor that is cytoplasmically activated, possibly through interaction with the interferon receptor. It associates with activated STAT1 and STAT2 to form an ISGF3 complex that is translocated into the nucleus.

Synonyms

Interferon regulatory factor 9; IRF-9; IFN-alpha-responsive transcription factor subunit; ISGF3 p48 subunit; Interferon-stimulated gene factor 3 gamma; ISGF-3 gamma; Transcriptional regulator ISGF3 subunit gamma; IRF9; ISGF3G

Gene Name

IRF9

Gene ID

10379

UniProt

Q00978

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human IRF9 recombinant protein (Position: M1-K110) . Human IRF9 shares 87.3% amino acid (aa) sequence identity with mouse IRF9.

Clonality

Polyclonal

Tissue Specificity

Leukocytes.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cell Adhesion, Cell Type Markers, Cytoskeleton/ECM, Hematopoietic Progenitors, Immunology, Integrins, Myeloid, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta) . Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

References & Citations

1. Fu, X.-Y., Kessler, D. S., Veals, S. A., Levy, D. E., Darnell, J. E., Jr. ISGF3, the transcriptional activator induced by interferon alpha consists of multiple interacting polypeptide chains. Proc. Nat. Acad. Sci. 87: 8555-8559, 1990. 2. Levy, D. E., Kessler, D. S., Pine, R., Darnell, J. E., Jr. Cytoplasmic activation of ISGF3, the positive regulator of interferon-alpha-stimulated transcription, reconstituted in vitro. 3. Suhara, W., Yoneyama, M., Yonekawa, H., Fujita, T. Structure of mouse interferon stimulated gene factor 3 gamma (ISGF3 gamma/p48) cDNA and chromosomal localization of the gene. J. Biochem. 119: 231-234, 1996.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

43696 MW

Observed Molecular Weight

44 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Interferon regulatory factor 9

Subcellular Location

Cytoplasm . Nucleus . Translocated into the nucleus upon activation by IFN-alpha/beta.

Protein Name

Interferon regulatory factor 9

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.